人肺腺癌细胞HCC78

细胞名称

人肺腺癌细胞HCC78

货物编码

BFN60808857

产品规格

T25培养瓶x1

1.5ml冻存管x2

细胞数量

1x10^6

1x10^6

保存温度

37℃

-198℃

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科研   2类

培养体系

90%DMEM+10%FBS+1%三抗

培养温度

37℃

二氧化碳浓度

5%

简介

人肺腺癌细胞HCC78取自65岁男性，该细胞源于DSMZ。

注释

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Part of: MD Anderson Cell Lines Project.

Doubling time: ~60-70 hours (DSMZ).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Deep exome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: Protein expression by reverse-phase protein arrays.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

Miscellaneous: Direct author submission of STR profile from Girard L. and Gazdar A.F.

基因突变

SLC34A2-ROS1 gene fusion (PubMed=18083107; PubMed=25485619).

Homozygous for CDKN2A p.Arg58Ter (c.172C>T) (p.Pro72Leu; c.215C>T) (ClinVar=VCV000376310) (CCLE).

Homozygous for TP53 p.Ser241Phe (c.722C>T) (ClinVar=VCV000012359) (CCLE; Cosmic-CLP).

HLA信息

STR信息

Amelogenin        X,Y

CSF1PO        11,12

D1S1656        15.3,17.3

D2S441        11,14

D2S1338        17,21

D3S1358        16,17

D5S818        11,12

D7S820        9

D8S1179        13,14

D10S1248        14,16

D12S391        19,21

D13S317        8,12

D16S539        11,13

D18S51        12,16

D19S433        16,16.2

D21S11        29,30

D22S1045        15,16

DYS391        12

FGA        21,22

Penta D        7,11

Penta E        11,12

TH01        7,9.3

TPOX        8,11

vWA        15,18

参考文献

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005

Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.

Characterization of human cancer cell lines by reverse-phase protein arrays.

Cancer Cell 31:225-239(2017)

PubMed=30038707; DOI=10.18632/oncotarget.25642

Du L., Zhao Z., Suraokar M., Shelton S.S., Ma X., Hsiao T.-H., Minna J.D., Wistuba I., Pertsemlidis A.

LMO1 functions as an oncogene by regulating TTK expression and correlates with neuroendocrine differentiation of lung cancer.

Oncotarget 9:29601-29618(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747

Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3

Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Nature 569:503-508(2019)