品牌 | 其他品牌 | 货号 | BFN60805937 |
---|---|---|---|
规格 | T25培养瓶x1 1.5ml冻存管x2 | 供货周期 | 现货 |
主要用途 | 仅供科研 | 应用领域 | 医疗卫生,生物产业 |
细胞名称 | 小鼠结缔组织L细胞株929克隆株L-929 | ||
货物编码 | BFN60805937 | ||
产品规格 | T25培养瓶x1 | 1.5ml冻存管x2 | |
细胞数量 | 1x10^6 | 1x10^6 | |
保存温度 | 37℃ | -198℃ | |
运输方式 | 常温保温运输 | 干冰运输 | |
安全等级 | 1 | ||
用途限制 | 仅供科研 1类 |
培养体系 | DMEM高糖+10%FBS+1%双抗 | ||
培养温度 | 37℃ | 二氧化碳浓度 | 5% |
简介 | 小鼠结缔组织L细胞株929克隆株L-929是最早建立的连续培养物之一,L细胞源自100天的雄性C3H/An小鼠的皮下网眼状空隙和脂肪组织。APRT+,HPRT+。 | ||
序列突变 | / | ||
注释 | Selected for resistance to: ChEBI; CHEBI:28748; Doxorubicin (Adriablastin; Adriamycin) | ||
STR信息 | / | ||
参考文献 | PubMed=4471172; DOI=10.1007/BF02615345 Ham R.G. Nutritional requirements of primary cultures. A neglected problem of modern biology. In Vitro 10:119-129(1974)
PubMed=6298990; DOI=10.1016/0378-1135(82)90056-6 McPhee D.A., Parsonson I.M., Della-Porta A.J. Comparative studies on the growth of Australian bluetongue virus serotypes in continuous cell lines and embryonated chicken eggs. Vet. Microbiol. 7:401-410(1982)
DOI=10.4051/ibc.2010.2.4.0014 Yoshino K., Saijo K., Noro C., Nakamura Y. Development of a simple method to determine the mouse strain from which cultured cell lines originated. Interdisciplinary Bio Central 2:1-9(2010)
DOI=10.5897/IJBMBR2013.0154 Iloki Assanga S.B., Gil-Salido A.A., Lewis Lujan L.M., Rosas-Durazo A., Acosta-Silva A.L., Rivera-Castaneda E.G., Rubio-Pino J.L. Cell growth curves for different cell lines and their relationship with biological activities. Int. J. Biotechnol. Mol. Biol. Res. 4:60-70(2013)
PubMed=23430347; DOI=10.1007/s10616-013-9545-7 Almeida J.L., Hill C.R., Cole K.D. Mouse cell line authentication. Cytotechnology 66:133-147(2014)
PubMed=31220119; DOI=10.1371/journal.pone.0218412 Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B., Gollin S.M., Lewis D.W., Reid Y.A. Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines. PLoS ONE 14:E0218412-E0218412(2019) |
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